LIVE CELL IMAGING

Fluorescence microscopy
 an integral part of modern cell biology
Staining required to image different cell organelles
" …although many small molecule inhibitors used in cell biology research are also highly fluorescent, increasing background fluorescence can generate extremely cytotoxic breakdown products"
STAIN LIMITATIONS

  • Signal strength intensity
  • Phototoxicity
  • Invasive
  • Bleaching of samples
  • Cytotoxicity

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