LIVE CELL IMAGING
Fluorescence microscopy
an integral part of modern cell biology
an integral part of modern cell biology
Staining required to image different cell organelles
" …although many small molecule inhibitors used in cell biology research are also highly fluorescent, increasing background fluorescence can generate extremely cytotoxic breakdown products"

STAIN LIMITATIONS
- Signal strength intensity
- Phototoxicity
- Invasive
- Bleaching of samples
- Cytotoxicity